食品工业科技2013，Vol.34Issue(8)：108-110,3.
运用Real-time quantification PCR方法建立副溶血性弧菌在即食虾中的生长预测模型
Establishment of Vibrio parahaemolyticus growth predictive model by Real-time quantification PCR on ready-to-eat Shrimp
摘要
Abstract
The object was to establish growth predictive model of Vibrio parahaemoiyticus on ready-to-eat shrimp by Real-time quantification PCR method. Firstly we constructed the standard piasmid and established the standard curve by 10-fold dilution. Secondly we detected the number of Vibrio parahaemoiyticus on shrimp by real-time qPCR method. At last we established the growth predictive model of Vibrio parahaemoiyticus on shrimp stored at 37℃. We compared this model with a traditional predictive model which used the data obtained by construction of microbiological methods. The results showed that it could establish Gmopertz model,Logistic model and Richards model based on the real-time qPCR method and construction of microbiological method (R2>0.9). Establishment of a microbial predictive model by real-time qPCR method will be a trend of predictive microbiology field in the future,because of the advantage of real-time qPCR methods such as time-saving,labar-saving and accurate.关键词
Real-time quantification PCR/副溶血性弧菌/生长预测模型Key words
Real-time quantification PCR/ Vibrio parahaemoiyticus/growth predictive model分类
轻工纺织引用本文复制引用
彭织云,王敬敬,唐晓阳,潘迎捷,赵勇..运用Real-time quantification PCR方法建立副溶血性弧菌在即食虾中的生长预测模型[J].食品工业科技,2013,34(8):108-110,3.基金项目
国家自然科学基金面上项目(31271870) （31271870）
上海市科委部分地方院校能力建设项目(11310501100) （11310501100）
上海市科委科技创新行动计划项目(12391901300) （12391901300）
上海市科委工程中心建设项目(11DZ2280300). （11DZ2280300）
