食品工业科技2013，Vol.34Issue(8)：183-186,4.
一株木薯纤维素分解菌的发酵条件和产酶特性研究
Study on the fermentation process and the characteristics of enzyme from cellulase-producing bacteria for the cassava
摘要
Abstract
The fermentation process and characteristics of the enzyme was studied on a cellulase-producing bacteria isolated from soil. The cellulase-producing process were optimized by orthogonal experiments, the optimal fermentation process was pH4.0,concentration of cassava in the culture solution 25g/L,bacteria inoculation 1.5% (V/V), shake culture for 56h in 33℃,the CMCase activity under these conditions achieved 16.982U/mL. The cell concentration attained the highest when the cellulase-producing bacteria was cultured for 24h in broth medium. The characteristics of the enzyme was confirmed,the optimal reaction temperature was 50℃.the optimal pH was 5.0,the optimal time was 60min.关键词
纤维素分解菌/木薯纤维/发酵条件/CMC酶活/酶的特性Key words
cellulase-producing bacteria/ cassava fibre/ fermentation process/ CMCase activity/ characteristics of the enzyme分类
轻工纺织引用本文复制引用
张会香,杨世军,向黎,陈昌朋..一株木薯纤维素分解菌的发酵条件和产酶特性研究[J].食品工业科技,2013,34(8):183-186,4.基金项目
广西教育厅科研项目(200807LX275). （200807LX275）
