食品工业科技2013，Vol.34Issue(8)：187-191,5.
酶解星虫水溶性蛋白制备降血压肽的工艺研究
Study on the preparation of antihypertensive peptides from water-soluble protein by enzymolysis
摘要
Abstract
Phascolosoma esculenta water-soluble protein hydrolyzed by pepsin,alkali protease,trypsin,neutral protease,and papain were studied. The hydrolysis condition of pepsin was optimized,with the ACE inhibiting rate as the major indicator,to:pH1.8,37℃ ,E/S 0.3% (w/v) and 4h. Under the optimized condition of pepsin hydrolysis,the IC50 of hydrolysate was 1.85mg/mL. The activity of ACE inhibitor in vivo was further investigated with spontaneously hypertensive rats (SHR) and results showed that blood pressure start to decrease significantly after 1h of oral administration and the average value of SBP reduced 17mmHg in 8h.关键词
可口革囊星虫/酶解/降血压肽/自发高血压大鼠Key words
Phascolosoma esculenta/ enzymolysis / antihypertensive peptide/ spontaneously hypertensive rats (SHR)分类
轻工纺织引用本文复制引用
杜磊,方明,吴洪喜,黄振华,李鹏,谢静莉..酶解星虫水溶性蛋白制备降血压肽的工艺研究[J].食品工业科技,2013,34(8):187-191,5.基金项目
浙江省海洋生物技术产业科技创新团队(2010R50029) （2010R50029）
中央高校基本科研业务费 （）
浙江省近岸水域生物资源重点实验室重点开放课题(J2011001). （J2011001）
