郑州轻工业学院学报（自然科学版）2013，Vol.28Issue(1)：11-15,5.DOI:10.3969/j.issn.2095-476X.2013.01.003
牛排菇SB65菌株液体培养基和培养条件的优化
Optimization of fermentation liquid medium and culture conditions of portabella mushroom (Agaricus brunnescens) SB65 strain
摘要
Abstract
The liquid medium and culture conditions of portabella mushroom(Agaricus brunnescens) SB65 strain were optimized by single-factor experiments and uniform design. The optimized medium were as fol-lows( w/v) :sucrose 5. 5% , yeast extract 1% , MgSO4·7H2O 0. 05% , KH2PO4 1% , NaCl 0. 714 7% , CaCl2·2H2O 1% and the optimized culture conditions were as follows:initial pH =7. 2,culture temperature 28℃ ,culture period 12 d,rotation rate 160 r/min,medium volume 50 mL per 250 mL flask and seed volume 8% (v/v). Under the optimized medium and culture conditions, the biomass of tested strain was 4.579 g/L.关键词
牛排菇/液体菌种培养基/菌丝体生物量/培养条件Key words
portabella mushroom ( Agaricus brunnescens ) / liquid spawn medium/ biomass/ culture condition分类
农业科技引用本文复制引用
何培新,秦慧迪,安正方,李政伟..牛排菇SB65菌株液体培养基和培养条件的优化[J].郑州轻工业学院学报（自然科学版）,2013,28(1):11-15,5.基金项目
河南省科技攻关项目(122102110134) （122102110134）
