食品工业科技2013，Vol.34Issue(5)：184-188,5.
响应面法优化红雪茶中类胡萝卜素的提取工艺
Optimization of extraction conditions of carotenoids from Lethariella spp.by response surface methodology
摘要
Abstract
Based on Box-Behnken center composite design within Design-Expert7.0 software and response surface results,the optimal extraction process for carotenoids from Lethariella spp.were determined and a regression model was established.On the basis of single factor tests,using absorbance values in a specific wavelength of carotenoids as response value, three key factors (solid - liquid ratio, extraction temperature and time) were selected as independent variables and acetone as extraction solvent.The results showed that the optimal extraction process of carotenoids from Lethariella spp.was obtained as followed:solid-liquid ratio of 1:3(g/mL),extraction temperature of 52℃,and extraction time of 20min.Under these conditions, the extraction of carotenoids from Lethariella spp.absorbance was 0.3682, i.e.22.092μg from per g Lethariella spp.raw materials.The results showed that regression equation fit well with experimental data because of a good accordance between the predicted and observe values.Meanwhile the optimized technology was stable and viable with minor error and good degree.关键词
天然色素/红雪茶/类胡萝卜素/响应面分析Key words
natural pigments/ Lethariella spp./ carotenoids/ response surface analysis分类
轻工纺织引用本文复制引用
杨青松,赵艳,王莹,熊勇,邓芳轶..响应面法优化红雪茶中类胡萝卜素的提取工艺[J].食品工业科技,2013,34(5):184-188,5.基金项目
国家自然科学基金项目(31060031) （31060031）
云南省教育厅基金一般项目(2010Y433) （2010Y433）
云南省本科教学实验生物技术示范中心项目(0205-2001015209) （0205-2001015209）
云南民族大学青年科学基金(09QN05). （09QN05）
