食品工业科技2013，Vol.34Issue(5)：299-303,5.
HPLC法测定云南省不同辣椒品种中辣椒碱及二氢辣椒碱的含量
Determination of capsaicine and dihydrocapsaicin in different kinds chills of Yunnan Province by high performance liquid chromatograph
摘要
Abstract
A high-performance liquid chromatograph(HPLC) method was developed for determinating contents of capsaicine and dihydrocapsaicin in different kinds chills in Yunnan Province.Chills was pretreated by the ultrasonic extraction,and capsaicine and dihydrocapsaicin were separated and assayed with reversed phase C18 column.Finally the determination was carried out using HPLC gradient elution method,using methanol-3‰ phosphate as the mobile phase(65%~90% ,0~30min)at a flow rate of 1.2mL/min.The detector wavelength was set at 280nm and the injection volume used was 20μL.The relative standard deviations of capsaicine and dihydrocapsaicin determinated were all less than 1% and the average recoveries was all above 99%.This method showed good separation effect and high stability, and could be used for determining the content of capsaicine and dihydrocapsaicin in different kinds of chills in Yunnan Province.The result demonstrated that the contents varied largely from different kinds,different degrees of maturity and different districts.关键词
高效液相色谱法/辣椒碱/二氢辣椒碱/辣椒Key words
high performance liquid chromatograph (HPLC)/ capsaicine/ dihydrocapsaicin/ chills分类
轻工纺织引用本文复制引用
刘录,陈海云,刘菲菲,姚元成,徐胜平,杨克沙,赵海云,曹建新..HPLC法测定云南省不同辣椒品种中辣椒碱及二氢辣椒碱的含量[J].食品工业科技,2013,34(5):299-303,5.基金项目
国家自然科学基金(30960048) （30960048）
昆明理工大学分析测试基金(2011419). （2011419）
