食品与发酵工业2013，Vol.39Issue(2)：41-44,4.
缬氨酸转氨酶拆分DL-缬氨酸的催化条件
Study on chiral resolution of DL-valine valine catalyzed by valine-pyruvate transaminase
摘要
Abstract
An engineered strain with catalytic activity of valine-pyruvate transaminase was used in chiral resolution of DL-valine.The effect of reaction conditions such as reaction temperature, pH, substrate mole ratio, substrate concentration and metal ions on enzyme activity was studied.Results showed that the optimal conditions were 45 ℃ and pH9.The optimal substrate ratio of L-valine to pyruvic acid was 1:8 ( mol: mol).The initial substrate concentration of DL-xaline and pyruvic acid were 0.6 mol/L and 2.4 mol/L respectively.Enzyme activity was improved by addition of 0.5 mmol/L Mg2+ and Na+.关键词
缬氨酸/转氨酶/拆分Key words
valine/ transaminase/ chiral resolution引用本文复制引用
张飞,魏涛,刘寅,韩亚伟,何培新..缬氨酸转氨酶拆分DL-缬氨酸的催化条件[J].食品与发酵工业,2013,39(2):41-44,4.基金项目
郑州轻工业学院博士基金资助项目(2010BSJJ020) （2010BSJJ020）
