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海洋低温β-半乳糖苷酶菌株筛选、鉴定及酶的分离纯化

崔爱萍 迟乃玉 张庆芳

食品与发酵工业2013,Vol.39Issue(2):92-96,5.
食品与发酵工业2013,Vol.39Issue(2):92-96,5.

海洋低温β-半乳糖苷酶菌株筛选、鉴定及酶的分离纯化

Isolation,identification and enzyme purification of a strain producing cold-activity β-galactosidase from marine

崔爱萍 1迟乃玉 2张庆芳3

作者信息

  • 1. 大连大学生命科学与技术学院,辽宁大连,116622
  • 2. 辽宁省海洋微生物工程技术研究中心,辽宁大连,116622
  • 折叠

摘要

Abstract

A strain CD6 from Huanghai sea mud was identified as Raoultella terrigena according to its morphology, taxonomical properties and molecular characterization.The purified β-galactosidase was obtained through dialysis, ultrafiltration, and column chromatography.Researches on the β-galactosidase showed that its optimal temperature was 25℃ and it was defined as cold-activity enzyme.

关键词

β-半乳糖苷酶/鉴定/分离纯化/低温酶

Key words

β-galactosidase/ identification/ purificayion/ cold-activity enzyme

引用本文复制引用

崔爱萍,迟乃玉,张庆芳..海洋低温β-半乳糖苷酶菌株筛选、鉴定及酶的分离纯化[J].食品与发酵工业,2013,39(2):92-96,5.

食品与发酵工业

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