沈阳农业大学学报2012,Vol.43Issue(6):725-730,6.
根癌农杆菌介导的高效水稻遗传转化影响因素研究进展
A Review on Influncing Factors of High Efficiency Agrobacterium tumefaciens-Mediated Rice Genetic Transformation
摘要
Abstract
Influencing factors of rice genetic transformation mediated by Agrobacterium were reviewed in this paper, and problems in process of transformation were discussed. Agrobacterium tumefaciens strains and vectors, rice genotypes, transform receptors, co -culture conditions, selectable marker genes and transformation processes are considered as indispensable links of high efficiency Agrobacterium tumefaciens -mediated rice genetic transformation. Many factors should be optimized in genetic transformation of rice before building up a high efficient Agrobacterium mediated transformation system for special varieties (lines) and tissue, as well as culture conditions.关键词
根癌农杆菌/遗传转化/高效/影响因素/水稻Key words
Agrobacterium tumefaciens/ genetic transformation/ high efficiency/ influencing factors/ rice (Oryza sativa L.)分类
农业科技引用本文复制引用
韩光明,李三和,陈志军,游艾青,陈温福..根癌农杆菌介导的高效水稻遗传转化影响因素研究进展[J].沈阳农业大学学报,2012,43(6):725-730,6.基金项目
国家转基因重大专项项目(2009zx08001-001B) (2009zx08001-001B)