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根癌农杆菌介导的高效水稻遗传转化影响因素研究进展

韩光明 李三和 陈志军 游艾青 陈温福

沈阳农业大学学报2012,Vol.43Issue(6):725-730,6.
沈阳农业大学学报2012,Vol.43Issue(6):725-730,6.

根癌农杆菌介导的高效水稻遗传转化影响因素研究进展

A Review on Influncing Factors of High Efficiency Agrobacterium tumefaciens-Mediated Rice Genetic Transformation

韩光明 1李三和 2陈志军 2游艾青 2陈温福3

作者信息

  • 1. 沈阳农业大学水稻研究所/农业部东北水稻生物与遗传重点实验室/北方超级粳稻育种教育部重点实验室,沈阳110161
  • 2. 湖北省农业科学院粮食作物研究所,武汉430064
  • 折叠

摘要

Abstract

Influencing factors of rice genetic transformation mediated by Agrobacterium were reviewed in this paper, and problems in process of transformation were discussed. Agrobacterium tumefaciens strains and vectors, rice genotypes, transform receptors, co -culture conditions, selectable marker genes and transformation processes are considered as indispensable links of high efficiency Agrobacterium tumefaciens -mediated rice genetic transformation. Many factors should be optimized in genetic transformation of rice before building up a high efficient Agrobacterium mediated transformation system for special varieties (lines) and tissue, as well as culture conditions.

关键词

根癌农杆菌/遗传转化/高效/影响因素/水稻

Key words

Agrobacterium tumefaciens/ genetic transformation/ high efficiency/ influencing factors/ rice (Oryza sativa L.)

分类

农业科技

引用本文复制引用

韩光明,李三和,陈志军,游艾青,陈温福..根癌农杆菌介导的高效水稻遗传转化影响因素研究进展[J].沈阳农业大学学报,2012,43(6):725-730,6.

基金项目

国家转基因重大专项项目(2009zx08001-001B) (2009zx08001-001B)

沈阳农业大学学报

OA北大核心CSCDCSTPCD

1000-1700

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