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玉米油中玉米赤霉烯酮的ELISA测定

孟卫芹 武玉香 唐世云 沈志强

现代食品科技2013,Vol.29Issue(6):1431-1433,3.
现代食品科技2013,Vol.29Issue(6):1431-1433,3.

玉米油中玉米赤霉烯酮的ELISA测定

Enzyme-linked Immunosorbent Assay for the Detection of Zearalenone in Corn Oil

孟卫芹 1武玉香 1唐世云 2沈志强1

作者信息

  • 1. 山东绿都生物科技有限公司,山东滨州256600
  • 2. 山东滨州畜牧兽医研究院,山东滨州256600
  • 折叠

摘要

Abstract

To know the performance of the kit,indirect competitive enzyme-linked immunosorbent assay(ELISA) was used to detect the residues of zearalenone in the 3 kinds of corn oil.The results showed that the linear equation was:y=-2.2x+0.66,r=0.9974,IC50=0.29 μg/kg.Quantitation of zearalenone was linear from 0.05 μg/kg to 4.05 ug/kg.The sensitivity was 0.05 ug/kg.The recovery rates in the corn oil were more than 85%.The sensitivity,repeatability and specificity ofthe method were satisfactory and could meet the need of detecting the residues of zenralenone in corn oil.

关键词

玉米油/玉米赤霉烯酮/酶联免疫检测法

Key words

com oil/ zearalenone/ enzyme linked immunosorbent assay(ELISA)

引用本文复制引用

孟卫芹,武玉香,唐世云,沈志强..玉米油中玉米赤霉烯酮的ELISA测定[J].现代食品科技,2013,29(6):1431-1433,3.

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