肉类研究2013，Vol.27Issue(4)：40-43,4.
DNA条形码技术在肉品防欺诈鉴别中的应用
Application of DNA Barcoding in Anti-Fraud Identification of Aquatic Products
摘要
Abstract
This paper reports the application of DNA barcoding techniques to identify whether or not the real species of insepction samples of import and export aquatic products such as fish is in accordance with the label discriptions.Genomic DNAs from aquatic products were extracted and amplified by PCR using the universal primer mitochondrial cytochrome C oxidase subunit Ⅰ (CO Ⅰ) gene.The amplified sequence was aligned with the corresponding ones published in the GenBank database and was submitted to the Barcode of Life Database (BOLD) for sequence analysis.Sixteen samples of aquatic products including fish and fishballs were detected by the proposed technique.One of them failed to be amplified by CO Ⅰ PCR whereas the species of the other 15 was successfully identified.Howoever,31.25％ of the identifed samples were not the same as described on their labels.As a rapid,simple and effective molecular identification technology,DNA barcoding can be directly applied to identify the species of meat products like fish.关键词
DNA条形码/冷冻鱼肉/动物源性食品/种类鉴定Key words
DNA barcoding/ frozen fish fillets/ animal derived food/ species identification分类
生物科学引用本文复制引用
邱德义,胡佳,刘德星,魏晓雅,刘国雄,岳巧云..DNA条形码技术在肉品防欺诈鉴别中的应用[J].肉类研究,2013,27(4):40-43,4.基金项目
"十二五"国家科技支撑计划项目(2012BAK11B05) （2012BAK11B05）
国家质量监督检验检疫总局科技计划项目(2012IK223)中山市科技计划项目(20123A298) （2012IK223）
