食品工业科技2013,Vol.34Issue(11):160-164,5.
稀有人参皂苷compound K转化菌株的筛选及转化条件优化
Screening of phytopathogenic fungi by minor ginsenoside compound K production and optimization of biotransformation conditions
摘要
Abstract
Twenty-two phytopathogenic fungi were screened with regard to their ability to produce minor ginsenoside compound K (C-K).Six fungi were detected to be able to convert major protopanaxadiol type saponins to ginsenoside C-K in the all tested strains,and the No.1.91 fungal strain which showed the most efficient biotransformation was morphologically identified as Oospora Wallr.The biotransformation pathways of ginsenoside Rb1 and Rb2 by No.1.91 fungal strain were:Rb1 → Rd → F2 → C-K,Rb2 → C-O→ C-Y.The following optimum conditions for transforming C-K by No.1.91 fungal strain were determined:the time of substrate addition,12h,pH5.0~6.0 and temperature 45℃.This study laid a certain foundation of the industrial preparation of minor ginsenoside C-K.关键词
人参皂苷/compound K/生物转化/筛选Key words
ginsenosides/ compound K/ biotransformation/ screening分类
轻工纺织引用本文复制引用
赵雪淞,孟月,陈星星..稀有人参皂苷compound K转化菌株的筛选及转化条件优化[J].食品工业科技,2013,34(11):160-164,5.基金项目
吉林省自然科学基金项目(200905106). (200905106)