食品工业科技2013，Vol.34Issue(11)：170-173,187,5.
L-亮氨酸生物合成关键酶基因的克隆和表达
Cloning and expression of key enzymes genes in biosynthesis of L-leucine
摘要
Abstract
The gene encoding the key enzyme in the biosynthetic pathway of L-leucine from Brevibacterium flavum ATCC14067 genome was obtained by PCR.The gene inserted into E.coli-C glutamicum shuttle expression plasmid pDXW-8 to construct a variety of recombinant plasmid,which was transformed into type strain C.glutamicum ATCC13032 and investigated its fermentation production of L-leucine.The shake flask fermentation experiment showed that L-leucine was not accumulated in the fermentation broth of the control strain,while L-leucine accumulation of 4.75g/L was detected in the fermentation broth of the genetically engineered strain ATCC13032/pDXW-8-leuA-ilvBNC.）11-0170-05关键词
关键酶/表达/谷氨酸棒状杆菌/L-亮氨酸Key words
key enzyme/ expression/ Corynebacterium glutamicum/ L-leucine分类
生物科学引用本文复制引用
张跃,张伟国..L-亮氨酸生物合成关键酶基因的克隆和表达[J].食品工业科技,2013,34(11):170-173,187,5.基金项目
国家863计划项目(2008AA02Z212). （2008AA02Z212）
