食品工业科技2013，Vol.34Issue(11)：182-187,6.
溶菌酶胁迫培养提高透明质酸分子量
Study on lysozyme stress raising the hyaluronic acid molecular weight
摘要
Abstract
Streptococcus equin physiological characteristics as the foundation,lysozyme stress was used to improve hyaluronic acid(HA) molecular weight.On the basis of single test,according to the Box-Benhnken desigh principles,the method of response surface method with 4 factors and 3 levels was established.Then the best lysozyme stress condition was determined by means of regression equation analysis.The result of response surface experiments showed that the optimal conditions was adding 0.2U/mL lysozyme at 9.0h in the seed medium,adding 0.6U/mL lysozyme at 7.0h in the fermentation medium.The molecular weight of HA could reach 2.44 x 106u.lt raised 34.1％ than control group.关键词
溶菌酶/响应面分析法/透明质酸Key words
lysozyme/ response surface method/ hyaluronic acid分类
轻工纺织引用本文复制引用
周晓惠,陈奕涵,周庆玮,黄慧娟,侯永泰,任彩霞,荣绍丰..溶菌酶胁迫培养提高透明质酸分子量[J].食品工业科技,2013,34(11):182-187,6.基金项目
上海市科促会联盟项目(LM201123) （LM201123）
上海市科委生物医药与农业领域重点项目(10431901900) （10431901900）
上海市科委产学研医项目(11DZ1921405). （11DZ1921405）
