食品工业科技2013，Vol.34Issue(12)：81-85,5.
QuEChERS-HPLC快速测定食品中七种食用合成色素
QuEChERS-high performance liquid chromatography for the determination of synthetic colorants in food
摘要
Abstract
A high performance liquid chromatographic method was developed for the simultaneous determination of 7 synthetic colours such as tartrazine,sunset yellow,ponceau 4R,amaranth,brilliant blue,carmoisine,allura red in bean and meat products.The analytes were extracted from the samples by ethanol-ammonia-water (volume ratio 7∶2∶1),and purified by a modified QuEChERS (quick,easy,cheap,effective,rigged,and safe)method,and then separated by a SunFireTM C18(250mmx4.6mm,5μm) after concentrating.Methanol-20mmol/L ammonium acetate was used as mobile phase with a gradient elution at the rate of 1mL/min and the detection wavelength was 410nm and 520nm.The interference of matrix was reduced by the matrix-matched calibration standard curves.The linear range was 0.5～10μg/mL with correlation coefficients from 0.9971 to 0.9999.When 0.5～10μg/mL standard solutions were added to blank samples,the average recoveries were 83.01％～108.84％and the relative standard deviations ranged from 4.53％～15.23％.The limits of detection were ranged from 0.1mg/L to 0.3mg/L and the limit of quantification from 0.3mg/kg to 0.4mg/kg.This method is simple,accurate and sensitive to screen synthetic colorants residue in bean and meat products.关键词
QuEChERS/高效液相色谱法/食用合成色素Key words
QuEChERS/ HPLC/ synthetic colorants分类
轻工纺织引用本文复制引用
刘丽,吴青,林凤英,邝敏华..QuEChERS-HPLC快速测定食品中七种食用合成色素[J].食品工业科技,2013,34(12):81-85,5.基金项目
广东省教育部产学研结合项目(2010B090400362) （2010B090400362）
广东省科技计划项目(2010A032000001-4). （2010A032000001-4）
