食品工业科技2013，Vol.34Issue(12)：284-288,5.
水解法制备石榴皮鞣花酸工艺研究
Study on preparation of ellagic acid by hydrolysis from pomegranate peel
杨笑笑 1邢晓平 2纪白慧 1卢婕 1曹玉华1
作者信息
- 1. 江南大学化学与材料工程学院,江苏无锡214122
- 2. 盐城工学院,江苏盐城224051
- 折叠
摘要
Abstract
Preparation of ellagic acid from pomegranate peel by hydrolysis was developed.Pomegranate peel powder was extracted with 60％ ethanol by means of ultrasonic-assisted extraction to obtain crude extract.After removal of lipophilic ingredients by extracting with petroleum ether,crude extract was extracted with ethyl acetate,and the ethyl acetate fraction was hydrolyzed to produce ellagic acid.With the ultra performance liquid chromatography-mass sPectrometry,the main ingredients in the ethyl acetate fraction were identified as castalagin,punicalagin,granatin A,corilagin,granatin B and ellagic acid,and the hydrolysis product was identified as ellagic acid.The hydrolysis process of the ethyl acetate fraction was monitored by capillary electrophoresis.It had been found that with the increase of hydrolysis time,the number of peaks in electropherogams was reduced,while the weight of ellagic acid increasing.With the orthogonal experiment,the influences of hydrolysis time,hydrolysis temperature,as well as acid concentration on the ellagic acid yield was investigated.It turned out that optimum conditions of hydrolysis were as follows:hydrolysis time of 6h,hydrolysis temperature at 100℃,and sulfuric acid of 1.0mol/L,with the ellagic acid yield of 32.7％.关键词
石榴皮/鞣花酸/酸水解/毛细管电泳/超高效液相色谱-质谱Key words
pomegranate peel/ ellagic acid/ acid hydrolysis/ CE/ UPLC-MS分类
轻工纺织引用本文复制引用
杨笑笑,邢晓平,纪白慧,卢婕,曹玉华..水解法制备石榴皮鞣花酸工艺研究[J].食品工业科技,2013,34(12):284-288,5.
