食品工业科技2013，Vol.34Issue(13)：276-279,288,5.
HPLC法分离鉴定樟子松树皮多酚研究
Study on separation and identification scotch pine bark polyphenols with HPLC method
摘要
Abstract
The HPLC method was employed to separate and determine polyphenols in park of pinus sylvestris var.mongolica.To optimize the separation conditions,detecting wavelength,mobile phase,mobile rate,column temperature and injecting volume were investigated.The optimal chromatographic separating condition was established:the detecting wavelength was 280nm,mobile phase was methanol-water (0.05％ TFA),mobile rate was 1.0mL/min,column temperature was 30℃ and injecting volume was 5μLThrough comparison with standard sample chart,p-Coumaric acid,catechin,caffeic acid,ruitin,chlorogenic acid,gallic acid,cinnamic acid was detected in park of pinus sylvestris var.mongolica.The high content of p-Coumaric acid and catechin in park of pinus sylvestris var.mongolica were analyzed.关键词
HPLC/樟子松树皮/多酚Key words
HPLC/ scotch pine bark/ polyphenol分类
轻工纺织引用本文复制引用
欧阳乐,王振宇,刘冉,梁薇薇,邓心蕊..HPLC法分离鉴定樟子松树皮多酚研究[J].食品工业科技,2013,34(13):276-279,288,5.基金项目
国家自然科学基金项目(31170510). （31170510）
