食品工业科技2013，Vol.34Issue(13)：292-295,4.
高效液相色谱法测定混菌发酵乳中乳糖、葡萄糖及半乳糖含量的变化
Changes of lactose, glucose and galactose contents in mixed fermented milk detected by HPLC method
摘要
Abstract
A high performance liquid chromatograph (HPLC)with refractive index detector for the determination of lactose,glucose and galactose and its changes in mixed fermented milk was established.Proteins from samples were precipitated with TCA reagent.The chromatographic separation was achieved on an Aminex HPX-87C column using ultrapure water as mobile phase at a flow rate of 0.3mL/min.The results demonstrated that good separation was obtained for the analytes.The calibration curves established showed good linearity over the concentration range of 0.5～10mg/mL.The correlation coefficient all reached more than 0.9999 and the detection limit varied from 0.012mg/mL to 0.016 mg/mL.The average recoveries for all three analytes ranged from 91.30％ to 103.28％ with a RSD between 0.49％ and 3.82％ (n =6).The method was applied to determine the sugars in fermented milk was reliable and accurate.关键词
高效液相色谱法/混菌发酵乳/乳糖/葡萄糖/半乳糖Key words
high pressure liquid chromatography(HPLC) / mixed fermented milk /lacose/ glucose/ galactose分类
轻工纺织引用本文复制引用
梁龙,牟光庆,陈历俊,姜铁民,吕雪峰..高效液相色谱法测定混菌发酵乳中乳糖、葡萄糖及半乳糖含量的变化[J].食品工业科技,2013,34(13):292-295,4.基金项目
国家科技部"863"计划(2011AA100903) （2011AA100903）
国家科技部"863"计划(2012AA101605). （2012AA101605）
