中国食品学报2013，Vol.13Issue(6)：9-14,6.
纯化工艺对金针菇多糖体外抗肿瘤活性的影响
Effect of Purification Processing on Antitumor Activity of Polysaccharides from Flammulina Velutipes in Vitro
摘要
Abstract
Objective:To investigate the effect of purification processing on antitumor activity of polysaccharides isolated from Flammulina velutipes.Methods:Three polysaccharides named as CFVP(crude Flammulina velutipes polysaccharides),DCFVP (deproteinazed crude Flammulina velutipes polysaccharides) and FVP2 (purified polysaccharide fraction 2of Flammulina velutipes) were prepared and co-incubated with human skin cancer cell line A375,human liver cancer cell line HepG-2,human throat cancer cell line HEp-2 and mouse melanoma cell line B16 with different concentrations of polysaccharides (15.6～500 μg/mL) in complete media or the media alone for 72h.The media were collected and centrifuged and the absorbance of supernatant was measured at 570 nm by a 96-well microplate reader.Inhibitory ratio and the least inhibition concentration of cell growth were calculated.Results:Three polysaccharides exerted proliferation inhibitory activity in a concentration-dependent manner.Highest inhibitory ratio to 4 tumor cell lines were observed as follow,64.6％,55.3％ and 29.3％ of CFVP (500 μg/mL) against A375,HepG-2 and HEp-2,as well as 69.2％ of FVP (500 μg/mL) to B16.The highest drug sensitivity were observed in CFVP,with half maximal inhibitory concentration to A375,HepG-2 and B16 were 177.8 μg/mL,396.6 μg/mL and 335.7 μg/mL respectively.Conclusion:In a word,CFVP with least purification processing showed the highest antitumor activity,which indicated the purification processing may have negative impact on the antitumor capacity of polysaccharides obtained from Flammulina velutipes.关键词
金针菇多糖/分离纯化/体外抗肿瘤活性Key words
Flammulina velutipes polysaccharide/ separation and purification/ antitumor activity in vitro引用本文复制引用
邹宇晓,廖森泰,何轩辉,王辉,刘学铭,施英,刘凡..纯化工艺对金针菇多糖体外抗肿瘤活性的影响[J].中国食品学报,2013,13(6):9-14,6.基金项目
广东省重大科技专项(2010B080701104) （2010B080701104）
