中国食品添加剂Issue(3)：59-66,8.
棉杆木聚糖碱法提取及酶解制备低聚木糖研究
Extraction of xylan from cotton stalks and preparation of xylooligosaccharides by enzymatic hydrolysis
摘要
Abstract
The conditions of water insoluble cotton stalks xylan extraction with alkali method was studied by single factor test and orthogonal experiments of L9 (34).The effects of pretreatment temperature,time of extraction,NaOH concentration and cotton stalks to NaOH solution ratio were studied.The yield of water insoluble xylan was up to 46.79％ when the conditon was 80C,4h,15％,1 ∶ 20.The xylanase L10904 were used in preparation of xylooligosaccharides by enzymatic hydrolysis and conditions were optimized via response surface Box-Benhnken central composite experimental design.A high yield of reducing sugarof 31.04％ was obtained when the conditions were as follows:substrate concentration 0.86％,enzyme amount 20.0U/mL,hydrolysis temperature 53℃ and hydrolysis time 240min.TLC analysis showed that the main components are xylobiose and xylotriose.关键词
棉杆/水不溶性术聚糖/低聚木糖/响应面分析Key words
cotton staiks / water insoluble xylan / xylooligosaccharides/ response surface analysis分类
化学化工引用本文复制引用
滕超,范园园,张鹏,李高达,李秀婷..棉杆木聚糖碱法提取及酶解制备低聚木糖研究[J].中国食品添加剂,2013,(3):59-66,8.基金项目
国家自然基金青年科学基金项目(No.31201449) （No.31201449）
北京市属高等学校人才强教计划资助项目(No.PHR 20110872). （No.PHR 20110872）
