中国食品添加剂Issue(3)：233-237,5.
高效液相色谱法测定蜜饯中的莱鲍迪苷A的含量
Determination of rebaudiodside a in preserved fruits by high performance liquid chromatography
摘要
Abstract
Stevioside is a hybrid natural sweetener and most widly used in food industy.Rebaudioside A is the highest sweeteness component in Stevioside.A high performance liquid chromatography (HPLC) method was established to detect the amount of rebaudiodside A (RA) in preserved fruit.A Kromasil NH2 column (4.6 mm ×250 mm,5μm) was used with acetonitrile-water (75:25,v/v) as mobile phase,analyzed by diode-array detector.The linear relationship was good within the concentration range of 0.025 ～ 0.50mg/mL,and the detection limit was 0.011 g/kg.In addition,the relative standard deviation of this method was 0.41％ (n =8),and the recoveries in preserved fruits of RA were ranged from 92.7％ to 110％.The method has high recovery rate and high sensitivity.It is more efficient and faster,and suitable for the analysis of the stevioside additive in preserved fruits.It is a technical support in the usage of national food safety standards.关键词
高效液相色谱/蜜饯/莱鲍迪苷A/甜菊糖苷Key words
high performance liquid chromatography (HPLC) / preserved fruit/ rebaudiodside A/ stevioside分类
轻工纺织引用本文复制引用
刘英,吴曙光,尹州,曹丽芬,吴广红..高效液相色谱法测定蜜饯中的莱鲍迪苷A的含量[J].中国食品添加剂,2013,(3):233-237,5.基金项目
浙江省质量技术监督系统科研计划项目(20110223). （20110223）
