食品工业科技2013，Vol.34Issue(14)：54-57,62,5.
荧光法检测鱼糜制品中大豆蛋白的研究
Detection of soybean protein in surimi products by fluorescently-labeled monoclonal antibody
摘要
Abstract
The usage of soybean proteins in surimi products is increasing and becomes a new problem for aquatic products processing.Thus,establishment of an effective detection method for soybean proteins is essential.In the present study,soybean trypsin inhibitor(STI) was purified from soybean meal by a series of pretreatments,including acetone,H2SO4 treatments,ammonium sulfate fractionation and sequential column chromatographies.For monoclonal antibody(A11-6) preparation,purified STI was used to immunize mouse and the serum was purified by a Protein G Sepharose affinity column.Heherobifunctional reagent N-succinimidyl3-2-pyridyldithiopropionate (SPDP) and dithiothreitol (DTT) were used successfully to crosslink anti-STI monoclonal antibody with R-phycoerythrin(R-PE).Western blot using this fluorescently-labeled antibody was then established and applied to analyze the existence of STI in surimi products.The limit of detection (LOD) of this method was 1.56μg/mL,which was slightly higher than traditional chemiluminescence method.This detection method is convenient,sensitive and safe in usage.关键词
R-藻红蛋白/荧光探针/单克隆抗体/鱼糜制品/大豆蛋白Key words
R-phycoerythrin/fluorescence probe/monoclonal antibody/surimi products/soybean protein分类
轻工纺织引用本文复制引用
江韬玲,沈建东,蔡秋凤,张凌晶,林岚,曹敏杰..荧光法检测鱼糜制品中大豆蛋白的研究[J].食品工业科技,2013,34(14):54-57,62,5.基金项目
福建省重大科研专项(2010NZ0001-3,2011NZ0002-1) （2010NZ0001-3,2011NZ0002-1）
福建省自然科学基金(2011J01227) （2011J01227）
厦门市科技计划项目(3502Z20113028). （3502Z20113028）
