食品工业科技2013，Vol.34Issue(14)：71-76,6.
荧光-化学计量学法快速检测牛奶中磺胺甲基异恶唑残留
Rapid analysis of sulfamethoxazole residue in milk based on fluorescence spectroscopy coupled with chemometrics
摘要
Abstract
Antibiotic residues in animal tissues and products could bring risks to the health of consumers.The commonly employed methods for detecting antibiotic residues often involve time-consuming protocols,whereas the fluorescence method was characterized as rapid and sensitive.In this study,a method had been developed for rapid screening of sulfamethoxazole residues in milk with fluorescence spectroscopy and the chemometrics tools.The established model was applied to 6 brands of commercial milk including pasteurized and UHT milk(whole,skimmed,semi-skimmed,high calcium).The results showed a significant discrimination by partial least squares discriminant analysis (PLS-DA) between the negative and positive milk samples with a 3.12％ of false negative probability,which was below 5％ required by the European Union.And quantification could be acquired by further analysis with partial least squares(PLS) with a recovery between 87.9％～131.6％.The correlation coefficient was 0.9992 in the linear range of 0～210μg/kg,and the detection capability(CCβ) and detection limit(CCα) were 2.54μg/L and 5.07μg/L,respectively.The average relative standard error of the prediction model was 3.24％.This method showed potential in rapid screening and analysis of sulfamethoxazole residues in milk.关键词
荧光光谱/化学计量学/磺胺类抗生素/牛奶Key words
fluorescence/ chemometrics/ sulfonamide/ milk分类
轻工纺织引用本文复制引用
张燕萍,冯仕云,周鹏,刘小鸣..荧光-化学计量学法快速检测牛奶中磺胺甲基异恶唑残留[J].食品工业科技,2013,34(14):71-76,6.基金项目
国家自然科学基金项目(30901128). （30901128）
