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木豆叶球松素的超声提取工艺及其抗氧化活性研究

付丽楠 袁肖寒 顾成波 蔡曼 祖元刚

食品工业科技2013,Vol.34Issue(14):224-228,5.
食品工业科技2013,Vol.34Issue(14):224-228,5.

木豆叶球松素的超声提取工艺及其抗氧化活性研究

Study on optimization of ultrasonic-assisted extraction technology and its antioxidant activity of pinostrobin from pigeonpea leaves

付丽楠 1袁肖寒 2顾成波 3蔡曼 1祖元刚1

作者信息

  • 1. 东北林业大学森林植物生态学教育部重点实验室,黑龙江哈尔滨150040
  • 2. 东北林业大学林学院,黑龙江哈尔滨150040
  • 3. 东北农业大学生命科学与生物技术研究中心,黑龙江哈尔滨150030
  • 折叠

摘要

Abstract

Based on single-factor experimental results,effects of four parameters including methanol concentration,extraction time,extraction temperature and ratio of solid to liquid on the extraction yield of pinostrobin from pigeonpea leaves were optimized by orthogonal test design,and the antioxidant capacity of methanol extract (ME) was evaluated by 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging ability.Optimal extraction conditions were obtained as follows:methanol concentration 80%,extraction time 60min,extraction temperature 50℃ and solid-liquid ratio 1∶40(g/mL).Under optimum conditions,the extraction yield of pinostrobin reached 1.832mg/g,and ME of pigeonpea leaves exhibited good antioxidant activity with an IC50 value of 0.281mg/mL.Ultrasonic-assisted extraction technology established in the study possessed the advantages of high efficiency and ease of operation,which was suitable for the extraction of pinostrobin from pigeonpea leaves.

关键词

木豆叶/球松素/正交设计/超声提取/抗氧化

Key words

pigeonpea leaves/pinostrobin/orthogonal design/ultrasonic-assisted extraction/antioxidant activitiy

分类

轻工纺织

引用本文复制引用

付丽楠,袁肖寒,顾成波,蔡曼,祖元刚..木豆叶球松素的超声提取工艺及其抗氧化活性研究[J].食品工业科技,2013,34(14):224-228,5.

基金项目

中央高校基本科研业务费专项资金资助(DL13CA02) (DL13CA02)

公益性行业科研专项经费项目(201204601) (201204601)

哈尔滨市科技创新人才专项资金(2011RFQXS074). (2011RFQXS074)

食品工业科技

OA北大核心CSCDCSTPCD

1002-0306

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