食品工业科技2013，Vol.34Issue(14)：278-282,286,6.
蒸汽闪爆结合碱溶酸沉法提取高温花生粕中的蛋白质
Extraction of protein from high-temperature peanut meal using combination process with steam flash-explosion,alkaline-extraction and acid-precipitation
摘要
Abstract
A combined process with steam flash-explosion,alkaline-extraction and acid-precipitation was used to extract protein from high-temperature peanut meal.Through single factor and orthogonal test,the optimum conditions were figured out.Firstly,the high-temperature peanut meal was soaked in 0.3％ sulfuric acid solution at 60℃ for 2h,then drained it and washed with water to remove the acid solution remained in the surface of the meal.The pretreated meal was treated with steam flash-explosion at 1.6MPa for 5min.The protein in the steam flash-exploded meal was extracted by alkaline-extraction and acid-precipitation process.The optimal condition for alkaline-extraction was solid to water ratio:1∶12(g/mL),pH9.5,60℃ and 2h.Under these conditions,the protein extraction rate reached 52.6％,which was 10.8％ higher than conventional alkaline extraction and acid-precipitation process.The water holding capacity,emulsifying activity index,foaming capacity and foaming stability of the protein extracted using this combined process were significantly improved,which increased by 67.1％,141.0％,131.3％ and 107.4％ respectively.Results showed that the combined process with steam flash-explosion,alkaline-extraction and acid-precipitation was appropriate for extracting protein from high-temperature peanut meal,which could increase the protein extraction rate and also improve the functional properties.关键词
高温花生粕/花生蛋白/蒸汽闪爆/碱溶酸沉Key words
high-temperature peanut meal/ peanut protein/ steam flash-explosion/ alkaline-extraction and acid-precipitation分类
轻工纺织引用本文复制引用
章玉清,杨瑞金,张文斌,华霄,赵伟..蒸汽闪爆结合碱溶酸沉法提取高温花生粕中的蛋白质[J].食品工业科技,2013,34(14):278-282,286,6.基金项目
国家863计划(2013AA102103) （2013AA102103）
江苏省自然基金项目(SBK201240610). （SBK201240610）
