沈阳农业大学学报2013，Vol.44Issue(2)：172-177,6.DOI:10.3969/j.issn.1000-1700.2013.02.008
兔卵母细胞体外成熟、体外受精及受精卵体外培养条件的研究
IVM,IVF of Follicular Oocytes and IVC of in Vitro Fertilized Oocytes in Rabbit
王辉田 1罗光彬 1王忠彬 1孙超 1陈思 1吴宏平 1王娇娇1
作者信息
- 1. 沈阳农业大学畜牧兽医学院,沈阳110161
- 折叠
摘要
Abstract
In order to establish a perfect in vitro production system of rabbit embryo,We explored the effects of the TCM-199,M16 and KSOM three different basic nutrient solutions on the in vitro maturation of rabbit oocytes,and the upstream method and direct washing method of sperm washing processing methods treating semen of rabbit oocyte in vitro fertilization and different concentrations of L-glutamine on the influence of the rabbit zygote.The results showed that TCM-199 as the foundation nutrient solution in vitro maturation rate (72.29％) was statistically higher than that in the M16 mature rate (59.11％) and KSOM mature rate (49.57％)(P ＜ 0.05).Upstream method processing sperm in vitro fertilized oocytes mother cell of the cleavage rate (64.89％)and segmentation growth rate (18.56％) were higher than direct washing method (51.9％,11.97％)(P＜0.05).0.5mmol·L-1 L-glutamine fertilized oocytes nutrient solution 2-4 cell growth rate (46.55％),4-8 cell growth rate (39.20％),8-16 cell growth rate (30.40％),mulberry embryo growth rate (26.65％),were significantly higher than those with 1 mmol·L-1 L-glutamine fertilized oocytes nutrient solution (were 38.83％,32.02％,25.14％,22.09％) and 2 mmol·L-1 L-glutamine fertilized oocytes nutrient solution group (divided into 33.96％,26.70％,21.43％,17.67％)(P ＜ 0.05),and the content of 0.5 mmol·L-1 L-glutamine fertilized oocytes nutrient solution set of blastula growth rate (15.52％) and with 1 mmol·L-1,2 mmol·L-1 L-glutamine fertilized oocytes nutrient solution blastula growth rate respectively (10.59％,7.17％)(P＜ 0.05).关键词
兔/卵母细胞/体外成熟/体外受精/胚胎发育Key words
rabbit/oocytes/ in vitro maturation/in vitro fertilization/embryonic development分类
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王辉田,罗光彬,王忠彬,孙超,陈思,吴宏平,王娇娇..兔卵母细胞体外成熟、体外受精及受精卵体外培养条件的研究[J].沈阳农业大学学报,2013,44(2):172-177,6.
