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海带多糖纯化及清除自由基活性研究

彭臻菲 方哲翔 刘敏 张其清

食品工业科技2013,Vol.34Issue(15):80-83,4.
食品工业科技2013,Vol.34Issue(15):80-83,4.

海带多糖纯化及清除自由基活性研究

Purification and radical scavenging effect of Laminaria japonica polysaccharide

彭臻菲 1方哲翔 2刘敏 2张其清2

作者信息

  • 1. 福州大学化学化工学院,福建福州350108
  • 2. 福州大学生物和医药技术研究院,福建福州350002
  • 折叠

摘要

Abstract

A purified polysaccharide of WPS-1-1 was obtained from crude extracts of Laminaria japonica by S-300 gel chromatography.The average molecular weight of WPS-1-1 was 112ku.Monosaccharides analysis revealed that WPS-1-1 was composed of fucose,xylose,galactose and mannose with a molar ratio of 6.9∶3.3 ∶ 1.5 ∶ 1.The results of infrared spectrum indicated that WPS-1-1 contained β-pyranose and sulfate group.In vitro,radical scavenging tests revealed that WPS-1-1 showed significant function of scavenging superoxide and hydroxyl radicals with IC50value of 0.35 and 1.0mg/mL.It could be a potential functional free-radical scavenger.

关键词

海带多糖/清除自由基/单糖组成

Key words

Laminaria japonica polysaccharide/ free radicals scavenging / monosaccharide composition

分类

轻工纺织

引用本文复制引用

彭臻菲,方哲翔,刘敏,张其清..海带多糖纯化及清除自由基活性研究[J].食品工业科技,2013,34(15):80-83,4.

基金项目

福州大学科研启动基金(2010-XY-16) (2010-XY-16)

福建省科技重大专项专题(2010NZ0001-1). (2010NZ0001-1)

食品工业科技

OA北大核心CSCDCSTPCD

1002-0306

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