食品工业科技2013，Vol.34Issue(15)：154-157,4.
基于rpoB靶基因的PCR法快速检测弓形菌
Detection of Arcobacter by polymerase chain reaction method based on the rpoB gene
摘要
Abstract
To establish a rapid,sensitive and specific method of polymerase chain reaction (PCR)for detection of Arcobacter species.PCR technique was used to amplify the rpoB gene of Arcobacter,and meanwhile the specificity and sensitivity of this method were valued.Only the target gene of Arcobacter was amplified at 205bp,while the fragments of other bacteria were not detected.The detection limit could reach 8.20 x 102cfu/mLThis PCR method was useful for the investigation of Arcobacter in food,with the advantages of simple operation,accurate reliability and good sensitivity.关键词
弓形菌/PCR/rpoB/检测Key words
Arcobacter/ PCR/ rpoB/ detection分类
轻工纺织引用本文复制引用
毕水莲,孟赫诚..基于rpoB靶基因的PCR法快速检测弓形菌[J].食品工业科技,2013,34(15):154-157,4.基金项目
中央高校基本科研业务费专项资金资助(2012ZZ0083) （2012ZZ0083）
广东省自然科学基金项目(10451064101005159). （10451064101005159）
