食品工业科技2013，Vol.34Issue(15)：158-161,165,5.
金黄色葡萄球菌噬菌体的分离筛选
Isolation and screening of Staphylococcus Aureus Phage
摘要
Abstract
The phages of Staphylococcus aureus were isolated from the sewage and stool sample after two times enrichment with host strain.The isolated phage was purified using the two layer plates and was identified by plaque observation and the electron microscopy.The titer and RTD values of the purified phage were evaluated and the host range was preliminary analyzed.The results showed that the plaques of the sensitive phage of Staphylococcus aureus were formed transparent clear zones and their sizes were 1～2mm in diameter.The titer of phage was 3.2 x 1010PFU/mL and the RTD value was 10-3.Electron microscope observation showed that the phage had an isometric head (30nm in diameter)and a tail (approximately 50nm in length).The phage could lyse the host bacteria and other two Staphylococcus aureus,but not Escherichia coil,Salmonella typhimurium and shigella dysenteriae.These data enabled us to explore more properties of Staphylococcus aureus phage.It would be a good agent for detecting foodborne Staphylococcus aureus and an efficient antimicrobial source for Staphylococcus aureus infection.关键词
金黄色葡萄球菌/噬菌体/分离筛选Key words
Staphylococcus aureus/ phage /isolation and screening分类
生物科学引用本文复制引用
李雪玲,冯惠玲,李锡平,黄玉敏,梁庆文,邓丽卿..金黄色葡萄球菌噬菌体的分离筛选[J].食品工业科技,2013,34(15):158-161,165,5.基金项目
华南农业大学大学生科技创新项目(L08068). （L08068）
