食品工业科技2013，Vol.34Issue(15)：180-183,189,5.
产亚油酸异构酶的乳酸菌的筛选
Screening of the lactobacillus producing linoleic acid isomerase
摘要
Abstract
The ability of linoleic acid isomerase(LAI) formation by 16 Lactobacillus strains was studied.Conjugated linoleic acid(CLA)in fermentation broth from the 16 strains was determined by UV spectrophotometry and gas chromatography.The putative LAI genes from the strains were PCR amplified and sequenced.The results indicated that the CLA production of 9 Lactobacillus strains were determined by UV spectrophotometry.Fermentation broth of 6 Lactobacillus strains contained the cis-9,trans-11CLA (50％ of total CLA) and trans-9,trans-11 CLA (50％ of total CLA) detected by gas chromatography.Myosin-cross-reactive antigen (MCRA) gene,which were closely associated with LAI,were successfully amplified and sequenced from Lactobacillus rhamnosus(ATCC 9595).关键词
共轭亚油酸/乳酸菌/亚油酸异构酶Key words
conjugated linoleic acid /lactic acid bacteria/linoleic acid isomerase分类
轻工纺织引用本文复制引用
李妍,魏霜,白卫滨,吴希阳..产亚油酸异构酶的乳酸菌的筛选[J].食品工业科技,2013,34(15):180-183,189,5.基金项目
国家863计划项目(2011AA100901). （2011AA100901）
