食品工业科技2013，Vol.34Issue(15)：242-245,4.
葡萄酒泥酵母超氧化物歧化酶分离提取工艺条件优化
Process optimization for extracting superoxide dismutase from waste wine yeast
摘要
Abstract
Waste wine yeast as the research object,the superoxide dismutase (SOD)was extracted from it by toluene method.Four extraction parameters including amount of toluene,react time,react temperature and pH used for single factor test,and optimization of process parameters through L9 (34) orthogonal test.The results showed that the optimal amount of toluene were 0.8mL/g,react time was 25min,react temperature was 50℃,pH was 8.0,under these conditions,the specific activity of SOD was 178.73U/mg.The process was simple and inexpensive and coude be applied to industrialized production.关键词
超氧化物歧化酶/甲苯/葡萄酒泥酵母/比活力Key words
superoxide dismutase(SOD) / toluene/ wine yeast/ specific activity分类
轻工纺织引用本文复制引用
杜娜,杨学山,韩舜愈,祝霞,付文力,王婧,盛文军,张波..葡萄酒泥酵母超氧化物歧化酶分离提取工艺条件优化[J].食品工业科技,2013,34(15):242-245,4.基金项目
甘肃省科技支撑计划项目(1104NKCA080). （1104NKCA080）
