现代食品科技2013，Vol.29Issue(8)：1779-1783,5.
苦白蹄石油醚提取物对肿瘤细胞增殖与细胞周期的影响
Effect of Petroleum Eether Extracts from Fomitopsis officinalis (Vill.∶Fr.)Bond.on Tumor Cell Proliferation and Cell Cycle
摘要
Abstract
The sulforhodamine B (SRB) assay was employed to evaluate the anti-tumor effect of petroleum ether extracts from Fomitopsis officinalis (Vill.:Fr.) Bond (PEFO) on SMCC-7721,SGC-7901,and Hep-2 cell lines in vitro.The results showed PEFO inhibited the growth of three kinds of cells in dose-dependent manner with the IC50 values on SMCC-7721,SGC-7901,and Hep-2 cells being of 58.74,74.13 and 83.18μg/mL,respectively.The SMCC-7721 cells exposed to PEFO obviously changed cell morphology and cellular ultrastructure.The SMCC-7721cell presented wrinkled nuclei,poor cell adherent growth and cell lysis,while mitochondria swell and a large number of vacuoles in cytoplasm were observed by transmission electron microscope.The flow cytometry results revealed that SMCC-7721cell decreased in S-phase and increased in G0/G1-phase,which arrested in G0/G1 phase.The study demonstrated that PEFO could inhibit cell proliferation in vitro,and the anti-tumor activity of PEPO might be achieved by inhibition of tumor cellular aerobic metabolism and intervention cell cycle.关键词
苦白蹄/细胞增殖/细胞周期/人肝癌细胞SMMC-7721Key words
Fomitopsis officinalis (Vill.:Fr.) bond/ cell proliferation/ cell cycle/ SMMC-7721 cell引用本文复制引用
李晓飞,胡姗姗,高君君,朱欣婷,刘云..苦白蹄石油醚提取物对肿瘤细胞增殖与细胞周期的影响[J].现代食品科技,2013,29(8):1779-1783,5.基金项目
贵州省科技厅、遵义医学院、遵义市科技局联合资金项目(黔科合J字LKZ[2010]48号) （黔科合J字LKZ[2010]48号）
