现代食品科技2013，Vol.29Issue(8)：1888-1892,5.
新型常压室温等离子体(ARTP)快速诱变高产苏氨酸的突变株
A Mutant Strain with High Threonine Yield Obtained by Rapid Mutation Using Novel Atmospheric and Room Temperature Plasmas
摘要
Abstract
To obtain mutants with high threonine productivity,atmospheric and room temperature plasmas (ARTP) mutation technique was used and combined with high throughput screening based on 48 well plates.Mutant 1905# was screened with high threoine productivity.Compared with original strain under the same fermentation conditions,the mutant 1905# showed better robustness and faster growth rate.The mutant showed similar optimum temperature (37 ℃) and pH (7.2) to the original strain,but it can tolerate lower pH.In the preliminary plate screening,the threonine concentration increased by 133.59％.For the flask fermentation process,the mutant 1905# showed higher glucose consumption and enhanced threonine productivity The threonine concentration by the mutant was increased by 99.6％ compared with that by the original.The 1905# mutant showed high genetic stability after 50 subcultures,offering theoretical reference for further increasing threonine industrialization level关键词
常压室温等离子体/苏氨酸/诱变/高通量筛选Key words
atmospheric and room temperature plasmas (ARTP)/ threonine/ mutation/ high throughput screening引用本文复制引用
蔡友华,李文锋,卢伟宁,郑明英,陆最青,张翀,李和平,邢新会..新型常压室温等离子体(ARTP)快速诱变高产苏氨酸的突变株[J].现代食品科技,2013,29(8):1888-1892,5.基金项目
清华大学自主科研计划项目(2011Z01019) （2011Z01019）
