现代食品科技2013，Vol.29Issue(8)：1999-2003,5.
豆奶甲醇提取物中抗氧化活性物质的鉴定
Identification of Antioxidants in Methanol Extracts from Soymilk
摘要
Abstract
The paper mainly investigated the antioxidaative capacity of the soymilk methanol extracts and analyzed the possible existing antioxidants.DPPH (1,1-Diphenyl-2-picrylhydrazyl radical) method and FRAP (Ferric reducing antioxidant power) method were used toanalyze the antioxidative capacity of soymilk methanol extracts.The total contents of phenolics and total flavonoids were identified by colorimetric method.The kinds and contents of bioactivities of flavonoids were identified by ultra-high performance liquid chromatography-quadruple time-of-flight-mass spectrometry(UHPLC-QTOF-MS).The results showed that the methanol extracts of soymilk owned antioxidative activity.The antioxidative values showed no difference between DPPH and FRAP methods.Total phenolics content (285.77±2.93 μg/mL) was higher than total flavonoids content (11.27±0.51 μg/mL) in soymilk methanol extract.The compounds were identified and quantified by UHPLC-QTOF-MS.Among five bioactivities identified,6,7,4'-Trihydroxyflavone and Rha-Ara-GlcA-SoyB showed positive correlation with their antioxidant values.However,genistein and daidzein perform negative correlation with antioxidant values.6,7,4'-Trihydroxyflavone may be the main compound attributing to antioxidation of the commercial soymilk.关键词
豆奶/抗氧化性/DPPH法/FRAP法/总酚含量/总黄酮含量Key words
soymilk/ antioxidant/ DPPH method/ FRAP method/ total phenolics content/ total flavonoids content引用本文复制引用
张华,SUMNER Lloyd,LEI Zhen-tian,HUHMAN David,WATSON Bonnie,付福友,于杰..豆奶甲醇提取物中抗氧化活性物质的鉴定[J].现代食品科技,2013,29(8):1999-2003,5.基金项目
国家自然科学基金资助项目(31171930) （31171930）
中央高校基本科研业务费专项资金(XDJK2013A014) （XDJK2013A014）
西南大学博士启动基金项目(SWU112016) （SWU112016）
