食品与发酵工业2013，Vol.39Issue(6)：53-58,6.
重组大肠杆菌产腈水解酶的培养条件优化
Optimization of culture conditions of recombinant Escherichia coil expressing nitrilase
摘要
Abstract
Methionine is not only an essential amino acid needed for human health,also an important feed additive.The recombinant E.coli BL21 (DE3) /pET-28b-NIT producing nitrilase was investigated.Based on the data obtained from the cultivation using shake flask,the fed-batch fermentation strategy for producing nitrilase by high cell density cultivation of recombinant E.coli in the 5-Liter fermentor was optimized and constructed.It indicated that the concentration of carbon source,nitrogen source and inducer were the important factors.Under the optimized conditions of 14 h fed-batch fermentation,the cell mass concentration and the specific activity reached 13.7 gDCW/L and 36 990 U/L,which was 4.3 and 5.1 times higher than previous optimization,respectively.关键词
重组大肠杆菌/腈水解酶/蛋氨酸/补料分批培养Key words
recombinant E.coli/ nitrilase/ methionine/ fed-batch fermentation引用本文复制引用
金利群,吴丛伟,柳志强,郑裕国,沈寅初..重组大肠杆菌产腈水解酶的培养条件优化[J].食品与发酵工业,2013,39(6):53-58,6.基金项目
浙江省自然科学基金项目(R3110155) （R3110155）
