食品与发酵工业2013，Vol.39Issue(6)：205-207,3.
ISAV实时荧光RT-PCR检测方法的建立
Real-time fluorescent RT-PCR method for detection of ISAV
摘要
Abstract
A real-time PCR method was developed for the specific,sensitive and rapid detection of ISAV(Infectious salmon anemia virus).A pair of specific primers and a specific probe was designed according to the ISAV gene conserved sequence.The real-time fluorescence PCR probe for detecting ISAV was established.The results showed that this method had good specificity and repeatability,the detection of ISAV minimum copy number was 13.Therefore,the study established the ISAV specific,sensitive and rapid real-time fluorescence PCR probe for fishery products.关键词
传染性鲑鱼贫血症病毒/实时荧光RT-PCRKey words
Infectious salmon anemia virus/ fluorescent PCR引用本文复制引用
杜雄伟,李叶,庞艳华..ISAV实时荧光RT-PCR检测方法的建立[J].食品与发酵工业,2013,39(6):205-207,3.基金项目
中央高校自主基金项目(DC12010304) （DC12010304）
