食品工业科技2013，Vol.34Issue(16)：49-52,56,5.
柱前衍生-反相高效液相色谱法测定栾果及黄山栾果中的氨基酸含量
Determination of amino acids in Koelrenteria paniculata fruit and Koelreuteria integrifoliola Merr fruit by reversed-phase high performance liquid chromatography with precolumn derivatization
摘要
Abstract
In order to determine the composition and contents of amino acids in Koelrenteria paniculata fruit and Koelreuteria integrifoliola Merr fruit from zhengzhou by reversed-phase high performance liquid chromatographic (RP-HPLC) method,an Ultimate Amino Acid column was used,and phenylisothiocyanate (PITC) was the precolumn derivatization agent.Elutions were carried out in a binary gradient mode.The Column temperature was 30℃,and detection wavelength was set at 254nm.Results showed that:Koelrenteria paniculata fruit and the Koelreuteria integrifoliola Merr fruit contained 17 kinds of amino acids,total amino acid content was 15.57％ and 17.53％ respectively,The recoveries of standard addition of amino acids were 80.5％～115.7％.The average recovery rate was 93.86％,glutanic acid content was the highest.In Koelrenteria paniculata fruit,the histidien content was the lowest,the isoleucine content in Koelreuteria integrifoliola Merr fruit was the lowest.This method was simple,accurate and efficient.Measurement results were satisfactory,which provided reference for the development and utilization of the wild resources.关键词
反相-高效液相色谱法/氨基酸/柱前衍生/栾果和黄山栾果Key words
reversed phase-high performance liquid chromatography/amino acids/precolumn derivatization/Koelrenteria paniculata fruit and Koelreuteria integrifoliola Merr fruit分类
农业科技引用本文复制引用
高向阳,游新侠,牛云辉..柱前衍生-反相高效液相色谱法测定栾果及黄山栾果中的氨基酸含量[J].食品工业科技,2013,34(16):49-52,56,5.基金项目
河南省重点学科建设基金项目(10466-X-082301). （10466-X-082301）
