食品工业科技2013，Vol.34Issue(16)：70-74,5.
苯甲酸免疫胶体金快速检测试纸条的研制
Development of colloidal gold immunochromatographic test strip of carboxybenzene
摘要
Abstract
Colloidal gold immune chromatography technology was applied in this study,the colloidal gold immunochromatographic test strip was developed in order to establish a rapid detection assay of benzoic acid.The colloidal gold with particle diameter 17nm was obtained by reducing gold chloride with sodium citrate,benzoic acid gold-labeled antibody was spotted on some chosen glass fiber made of colloidal gold combined pad,and the antigen of benzoic acid and Goat anti Rabbit lgG were coated on the nitrocellulose membrane to be the test line(T line) and the control line(C line).Test strips were constructed and the detection sensitivity was measured.Results showed that the detection limit of colloidal goldimmunochromatographic test strip was 100μg/L and the whole analysis process could be completed within 5～7min.With phenol,toluene,benzene acetic acid,phenylalanine and other drugs had no cross reaction:Results were in complete accord with enzyme-linked immunosorbent method.The method had simple operation,fast characteristics,strong specificity,high sensitivity.The colloidal gold immunochromatographic test strip could be widely used for On-site rapid detection and screening work and had great prospectfor commercial development.关键词
苯甲酸/胶体金/试纸条/快速检测Key words
carboxybenzene/ colloidal gold/ dipsticks/ rapid detection分类
轻工纺织引用本文复制引用
曾文亮,杨金易,王弘,沈玉栋,罗林,曾道平,徐振林,孙远明..苯甲酸免疫胶体金快速检测试纸条的研制[J].食品工业科技,2013,34(16):70-74,5.基金项目
农业部公益性行业专项(201003008-08) （201003008-08）
广东省教育部产学研项目(2011A090200029) （2011A090200029）
福建省科技计划重点项目(2012Y0003). （2012Y0003）
