食品工业科技2013，Vol.34Issue(16)：187-190,4.
植物乳杆菌活的非可培养状态的初步研究
Preliminary study on the viable but non-culturable state of Lactobacillus plantarum
摘要
Abstract
The 16S rDNA sequence of an unidentified lactic acid bacteria was measured by 16S rDNA sequence analysis method,and compared the gene sequences homology with the gene pool,and the bacterium was identified as Lactobacillus plantarum.Conditions to induce Lactobacillus plantarum into viable but nonculturable(VBNC) and the resuscitation conditions were studied.Results showed that when the bacterium was induced in the condition of aerobic,-20℃,in MRS liquid medium,pH5.5～6.2 the bacterium entered VBNC state in 12d.Lactobacillus plantarum could resuscitate from VBNC state when it was incubated in MRS liquid medium added with 6％ tween-80 and the MRS liquid medium added with nothing in 48h and 96h respectively.关键词
植物乳杆菌/16S rDNA/活的非可培养状态/诱导/复苏Key words
Lactobacillus plantarum/ 16S rDNA /viable but non-culturable /induce/ resuscitate分类
轻工纺织引用本文复制引用
金磊,王丽,钟青萍..植物乳杆菌活的非可培养状态的初步研究[J].食品工业科技,2013,34(16):187-190,4.基金项目
国家自然科学基金资助项目(31271956) （31271956）
国家自然科学基金青年项目(31000781). （31000781）
