食品与发酵工业2013，Vol.39Issue(7)：18-23,6.
芝麻香型白酒高温大曲嗜热功能菌的筛选与鉴定
Screening and identification of thermophilic functional bacteria from high-temperature daqu of sesame flavor liquor
摘要
Abstract
The high-temperature daqu of sesame flavor liquor was used as experimental samples.Thermophilic functional bacteria were isolated by culturing at 55 ℃.Through screening of transparent circle and assaying of the solid-state fermentation enzyme activity,protease production strains were isolated.The strains were identified with 16S rDNA gene phylogenetic analysis.The results revealed that 85 strains of thermophilic bacteria were isolated from samples.The neutral protease activity of M5 was higher than control strain,and the enzyme activity of M5 was 96.06 U/ g.The strain M5 was identified as Bacillus subtilis subsp.subtilis through molecular biology methods.The strain M5 can be used as microbial agents to produce high-temperature daqu of sesame flavor liquor.关键词
芝麻香型白酒/高温大曲/中性蛋白酶/筛选/鉴定Key words
sesame flavor liquor/ high-temperature daqu/ neutral protese/ screening/ identification引用本文复制引用
姚粟,张明娟,刘勇,信春晖,许玲,张柏林,程池..芝麻香型白酒高温大曲嗜热功能菌的筛选与鉴定[J].食品与发酵工业,2013,39(7):18-23,6.基金项目
国家科技支撑计划课题(2012BAK17B11) （2012BAK17B11）
