食品与发酵工业2013，Vol.39Issue(7)：88-93,6.
L-苯丙氨酸抗噬菌体生产菌的选育和应用
Screening of bacteriophage-resistant strain for L-phenylalanine production
周海岩 1刘龙 2堵国成 2陈坚2
作者信息
- 1. 浙江工业大学生物工程研究所,浙江杭州,310014
- 2. 江南大学生物工程学院,江苏无锡,214122
- 折叠
摘要
Abstract
To cope with the bacteriophage problem for improvement of L-Phe production,we isolated bacteriophage BP-1 from the contaminated fermentation broth of E.coli WSH-Z06 (pAP-B03) producing L-phenylalanine (LPhe).Through the treatment of WSH-Z06 with N-methyL-N'-nitro-N-nitrosoguanidine (NTG),selection for resistance to BP-1,transformation with plasmid pAP-B03,and examination for the capacity of L-Phe production,we obtained one mutant strain E.coli BR-42 (pAP-B03).In the presence of BP-1,E.coli BR-42 (pAP-B03) exhibited a satisfactory performance in cell growth and L-Phe production.It was confirmed that E.coli BR-42 (pAP-B03) was a potential L-Phe over-producer against bacteriophage BP-1.It could be an excellent substitute for its parent strain WSH-Z06 (pAP-B03) in L-Phe industry production.关键词
L-苯丙氨酸/大肠杆菌/噬菌体/NTG诱变/筛选Key words
L-Phenylalanine/ Escherichia coli/ bacteriophage/ NTG mutagesis/ screening引用本文复制引用
周海岩,刘龙,堵国成,陈坚..L-苯丙氨酸抗噬菌体生产菌的选育和应用[J].食品与发酵工业,2013,39(7):88-93,6.
