中国食品添加剂Issue(4)：205-208,4.
HPLC法测定不同产地火麻仁中五种脂肪酸含量
Simultaneous determination of five main fatty acids in cannabis semen by HPLC
摘要
Abstract
Objective:To establish HPLC method of simultaneous determination of five main fatty acids (α-linolenic acid,linoleic acid oleic acid,palmitic acid and stearic acid) in Cannabis semen,for quality control of Cannabis semen from different origins.Methods:The chromatography was carried out on an Agilent Eclipse XDB-C18 column (150 mm 4.6 mm,5μm) with methanol-water (v ∶ v =85 ∶ 15) as the mobile phase.The flow rate was 1.0mL/min and the detection wavelength was 210 nm.The linear ranges of methyl linolenate,methyl linoleate,methyl oleate,methyl palmitate and methyl stearate were 42 ～168μg (r =0.9998),67～267μg (r=0.9995),9.7～39μg (r=0.9995),23 ～ 94μg (r =0.9996) and 14 ～ 56μg (r =0.9997),respectively.The average recoveries were 99.5％ (RSD=0.65％,n=6),99.5％ (RSD=1.20％,n=6),99.2％ (RSD=0.41％,n=6),99.0％ (RSD =0.60％,n =6) and 99.5％ (RSD =0.61％,n =6),respectively.Conclusion:This method has good reproducibility.The Cannabis semen from Baoding has the highest fatty acids contents,and this experiment provid ed the reference for further development and usage of hemp seed oil.关键词
火麻仁/油脂/(-亚麻酸/亚油酸/高效液相色谱法Key words
cannabis semen/ oil/α-linolenic acid, linoleic acid, HPLC分类
轻工纺织引用本文复制引用
夏林波,朱江,蔡明宸,邓仕任..HPLC法测定不同产地火麻仁中五种脂肪酸含量[J].中国食品添加剂,2013,(4):205-208,4.基金项目
2015版中国药典一部科研立项(火麻仁药材及饮片) （火麻仁药材及饮片）
辽宁省教育厅科学技术研究项目(No.2008450). （No.2008450）
