中国食品学报2013，Vol.13Issue(7)：89-94,6.
乳糖酶水解乳糖条件的优化
Optimization of the Lactose Hydrolysis Conditions
摘要
Abstract
The effects of temperature,pH,the ratio of enzyme to substrate,time on milk lactose hydrolysis were discussed.The optimum hydrolysis conditions were based on single factor tests,and then the response surface experiments of three factors and three levels were designed according to the principles of Box-Benhnken center combination.A regression model about milk lactose hydrolysis and its factors was established.The optimum conditions for milk lactose hydrolysis by enzyme were 41.78 ℃,time 2.63 h and the ratio of enzyme to substrate was 0.192％.Under these conditions,the milk laetose hydrolysis was 76.1％.关键词
乳糖/乳糖酶/水解率Key words
laetose/ lactase/ hydrolysis rate引用本文复制引用
曹晓燕,潘道东..乳糖酶水解乳糖条件的优化[J].中国食品学报,2013,13(7):89-94,6.基金项目
宁波市重点科技创新团队(2012B82017) （2012B82017）
宁波市重大攻关项目(2011C11017) （2011C11017）
宁波市创新基金(2010C92024) （2010C92024）
