茶叶科学Issue(5)：420-428,9.
茶树雌蕊总蛋白质双向电泳分离体系的建立
Establishment of Two-dimensional Electrophoresis System for Pistil Proteome Analysis in Tea Plant
摘要
Abstract
In order to establish a proper two-dimensional gel electrophoresis (2-DE) protocol for proteomic study of tea plant pistils, several protein extraction methods, density of SDS-PAGE gel, IEF procedures and pH gradient of IPG strip were compared. The results showed the optimized system includes: total proteins of sample extracted using TCA/acetone method followed with clean up, separating the proteins with 17 cm pH 5~8 IPG strips, 13%SDS-PAGE, and CBB R-250 stain method. About 1 200 spots can be detected, and these proteins mainly distributed in the pH 5~8, MW 14~117 kD range. With good separation of the basic protein, this will be helpful to proteomic research in tea plant.关键词
茶树/雌蕊/蛋白质提取/双向电泳Key words
Camellia sinensis (L.) O. Kuntze/pistil/protein sample preparation/two-dimensional electrophoresis分类
农业科技引用本文复制引用
任燕,陈暄,房婉萍,李垚,黎星辉..茶树雌蕊总蛋白质双向电泳分离体系的建立[J].茶叶科学,2013,(5):420-428,9.基金项目
国家自然科学基金（30972403）、江苏高校优势学科建设工程资助项目 （）
