茶叶科学Issue(1)：79-86,8.
茶梗固态生料发酵产单宁酶研究
Tannase Production from Tea Stalks under Non-heat-treated Solid State Fermentation
摘要
Abstract
The tannase production from Aspergillus niger by non-heat-treated solid state fermentation were investigated using tea stalks as substrate. The results showed that non-heat-treated solid state fermentation stimulated the production of tannase by 3.2-fold, when compared to that of heat-treated group. Supplementation with tannic acid was confirmed to have no influence on tannase production. Meanwhile, additional inorganic salts, carbon and nitrogen sources showed synergistic effect on tannase formation. Sucrose supplementation obtained maximum tannase yield, but the enzyme productivity in the initial stage of fermentation was decreased. Additional ammonium chloride played an important role in tannase synthesis process. It not only increased tannase production, but also shortened the fermentation period observably. Moreover, being supplemented with 7% sucrose and ammonium chloride obtained highest tannase production (23.6 U·g-1). These results demonstrated that using tea stalks as substrate to produce tannase by non-heat-treated solid state fermentation is a good choice, which can provide the theory basis for further study of tannase production. Meanwhile, it will help to promote the large-scale exploitation of tea stalks and to increase the economic value of this agro-residue.关键词
农业废弃物/茶梗/单宁酶/生料固态发酵/黑曲霉Key words
agro-residue/tea stalks/tannase/non-heat-treated solid state fermentation/Aspergillus niger分类
轻工纺织引用本文复制引用
吴昌正,蔡慧农,倪辉,朱艳冰,杨远帆,肖安风..茶梗固态生料发酵产单宁酶研究[J].茶叶科学,2014,(1):79-86,8.基金项目
集美大学科研创新团队基金（2010A006）、李尚大基金项目（ZC2011014）资助 （）
