茶叶科学Issue(2)：180-187,8.
SNaPshot技术检测茶树SNP研究
Study of SNaPshot Detect SNP Markers in Tea Plant
摘要
Abstract
In order to increase the genotyping efficiency of tea SNPs and promote th e application of SNPs in tea genetic breeding investigation, the feasibility of SNaPshot in SNPs genotyping of tea plant was investigated. Ten SNPs were selected from previous experiment as target SNPs. Then, these SNPs were detected in different tea cultivars by SNaPshot technology. Six among 10 SNPs were successful detected, with an accuracy of 60%. The polymorphism diversity of these SNPs was also analyzed. The value of NA was 2, He ranged from 0.37 to 0.52, Ho ranged from 0.32 to 0.74, PIC ranged from 0.36 to 0.50. All these markers were shown coincide between`Longjing43`and its repeated test. The markers reported here will be useful for tea genetic linkage map construction and genetic diversity study. The SNaPshot technology will promote the genetic study and accelerate the breeding process in tea plant.关键词
茶树/SNPs/SNaPshot/遗传多样性/遗传图谱Key words
tea plant/SNPs/SNaPshot/genetic diversity/genetic linkage map分类
农业科技引用本文复制引用
张成才,谭礼强,王丽鸳,韦康,成浩..SNaPshot技术检测茶树SNP研究[J].茶叶科学,2014,(2):180-187,8.基金项目
现代农业产业技术体系建设专项（No. nycytx-23）、浙江省自然科学基金（X307603）、浙江省茶产业技术创新战略联盟专项 （）
