中外葡萄与葡萄酒Issue(5)：6-11,6.
葡萄籽中原花青素的提取与分离
The extraction and purification of proanthocyanidins in grape seed
摘要
Abstract
Organic solvents were used to extract proanthocyanidins from grape seed in this experiment and the extraction conditions were determined by orthogonal experiments as follows:the extraction solution pH value adjusted to 3, material liquid 1:12, 70% ethanol concentration, extraction temperature 70 ℃, extraction time 2 h, extracting 3 times. HPD100 resin was selected from eight kinds of macroporous adsorption resins by static experiment. The experiment results showed that the adsorption quantity and desorption rate of proanthocyanidins and the desorption liquid purity were high with HPD100 resin, good for the separation and purification of grape seed procyanidins. Dynamic experiment determined that the optimal concentration of sample loading was 8.0 mg/mL, pH value of 3, velocity of 3 BV/h. The desorption rate was the highest by 60%ethanol solution with the velocity of 2 BV/h. The purity of proanthocyanidins extracted by this technology was above 60%.关键词
葡萄籽/原花青素/柱层析/分离Key words
grape seed/proanthocyanidin/column chromatography/separate分类
化学化工引用本文复制引用
高德艳,胡文效,魏彦峰,李彦奎,张晶莹..葡萄籽中原花青素的提取与分离[J].中外葡萄与葡萄酒,2013,(5):6-11,6.基金项目
山东省科技发展计划项目（2012G0022114）葡萄籽生物活性成分分离技术集成及产业化研究。 （）
