中外葡萄与葡萄酒Issue(1)：6-11,16,7.DOI:10.13414/j.cnki.zwpp.2014.01.001
葡萄果实分支酸变位酶基因的克隆与表达分析
Cloning and expression of gene encoding chorismate mutase in grape berries
张晨 1陈灵婧 1李小溪 1王军1
作者信息
- 1. 中国农业大学食品科学与营养工程学院葡萄与葡萄酒研究中心，北京 100083
- 折叠
摘要
Abstract
Shikimate pathway connects primary carbohydrate metabolism with the biosynthesis of most secondary metabolites in plants. Chorismate Mutase (CM) is an entry enzyme from shikimate pathway into phenylpropanoid metabolism, which plays an important role in the accumulation of phenolic compounds in grape berries. In the present study, two CM cDNAs were cloned from grape berries (Vitis vinifera cv. Cabernet Sauvignon) and designated as VvCM1 and VvCM2. The cDNAs of VvCM1 and VvCM2 contained open reading frames of 741 bp and 963 bp, which encoded a polypeptide of 246 and 320 amino acid residues with a molecular mass of 27.06 kDa and 35.2 kDa, respectively. The DNA sequences corresponding to the two isogenes both contain four introns and located on the chromosome 4 and 14, respectively. The sequence homology comparison showed that VvCM1 and VvCM2 had high homology of the amino acid sequences with other plant CM from the GenBank. Analysis by Real time-PCR showed that VvCM1 and VvCM2 were expressed in all the tested tissues. The transcript abundance of VvCM1 was the highest in grape berries and VvCM2 had the highest transcript level in stems.关键词
分支酸变位酶/基因克隆/葡萄果实Key words
Chorismate Mutase/Gene expression/grape berry分类
农业科技引用本文复制引用
张晨,陈灵婧,李小溪,王军..葡萄果实分支酸变位酶基因的克隆与表达分析[J].中外葡萄与葡萄酒,2014,(1):6-11,16,7.
