粮油食品科技2015，Vol.23Issue(1)：63-66,4.
免疫亲和柱净化-高效液相色谱法同时检测小麦中脱氧雪腐镰刀菌烯醇及其衍生物
Simultaneous determination of deoxynivalenol and ramification in wheat by immune affinity column and high performance liquid chromatography
摘要
Abstract
The method to determine deoxynivalenol (DON),3-acetyl- deoxynivalenol (3-A-DON) and 15-acetyl-deoxynivalenol (15 -A -DON )in wheat simultaneously by immune affinity column clean-up and high performance liquid chromatography was established. The wheat samples were extrac-ted by ultrapure water,cleared up with the immuno-affinity column (IAC). After drying and constant volume,three toxins,DON、3-A-DON and15-A-DON,were detected simultaneously by high per-formance liquid chromatograph with UV detector,and compared with that by Liquid chromatography mass spectrometry. The result showed that the detection limit of method was 100μg/kg for DON,3-A-DON and 15-A-DON respectively. Recovery rates in wheat spiked with DON,3-A-DON,and 15-A-DON,ranged from 86. 6% to 96. 5%. The variation coefficient was less than 10%. This method was suitable for determination of DON,3 -A-DON,and 15 -A-DON in wheat,with the advantages of simplicity,rapidness,sensitivity and good selectivity,veracity and precision.关键词
免疫亲和柱/脱氧雪腐镰刀菌烯醇/3-乙酰基-脱氧雪腐镰刀菌烯醇/15-乙酰基-脱氧雪腐镰刀菌烯醇/高效液相色谱Key words
immune affinity column/deoxynivalenol(DON)/3-acetyl-deoxynivalenol/15-acetyl-deoxynivalenol/high performance liquid chromatography分类
医药卫生引用本文复制引用
王培,徐广超,尹丽梅,黎睿,谢刚..免疫亲和柱净化-高效液相色谱法同时检测小麦中脱氧雪腐镰刀菌烯醇及其衍生物[J].粮油食品科技,2015,23(1):63-66,4.基金项目
十二五国家科技支撑计划(2012AA101609) （2012AA101609）
