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机械预处理对酶解啤酒糟提取阿魏酰低聚糖的影响

张秋培 尤梦竹 蔡国林 曹钰

食品与发酵工业2015,Vol.41Issue(1):1-6,6.
食品与发酵工业2015,Vol.41Issue(1):1-6,6.DOI:10.13995/j.cnki.11-1802/ts.201501001

机械预处理对酶解啤酒糟提取阿魏酰低聚糖的影响

Effect of mechanical pretreatment on extraction of feruloyl oligosaccharides from brewer's spent grain by enzymatic hydrolysis

张秋培 1尤梦竹 2蔡国林 3曹钰2

作者信息

  • 1. 江南大学工业生物技术教育部重点实验室,江苏无锡,214122
  • 2. 江南大学生物工程学院,江苏无锡,214122
  • 3. 江南大学糖化学与生物技术教育部重点实验室,江苏无锡,214122
  • 折叠

摘要

Abstract

Mechanical pretreatment of brewer's spent grain (BSG) can change its tightness and the particle size,thus affecting enzymatic hydrolysis efficiency.With the increase of degree of crushing,enzymatic efficiency increase.The yield of feruloyl oligosaccharides (FOs) from untreated BSG by xylanase and cellulase were 31.89% and 33.41%,respectively.And after the synergy of xylanase and cellulase,the yield of FOs increased to 47.01%.The yield of FOs from mechanical treated BSG hydrolyzed by xylanase and cellulase were 39.49% and 50.36%,respectively.And after the synergy of xylanase and cellulase,the yield of FOs increased to 64.00%.The results indicated that pretreatment could improve the' efficiency of enzymatic hydrolysis,and the presence of cellulase and β-glucanase was beneficial to the effect of xylanase,thus improved the extraction efficiency of FOs from BSG and initially realized the high-value utilization of BSG.

关键词

啤酒糟/刀片粉碎/球磨/酶解/阿魏酰低聚糖

Key words

brewer's spent grain/ blade grinding/ ball milling/ enzymatic hydrolysis/ feruloyl oligosaccharides

引用本文复制引用

张秋培,尤梦竹,蔡国林,曹钰..机械预处理对酶解啤酒糟提取阿魏酰低聚糖的影响[J].食品与发酵工业,2015,41(1):1-6,6.

基金项目

国家自然科学基金项目(31171736) (31171736)

国家863计划(2013AA102109) (2013AA102109)

江苏省普通高等学校科研成果产业化推进项目(JHB2012-26) (JHB2012-26)

江苏省科技支撑计划(BE2012397) (BE2012397)

江苏高校优势学科建设工程资助项目 ()

江南大学糖化学与生物技术教育部重点实验室开放课题(KLCCB-KF201407) (KLCCB-KF201407)

食品与发酵工业

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